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M94A3294.TXT
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1994-10-25
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Document 3294
DOCN M94A3294
TI The expression of gag-pol and gag-pol-env sequences of HIV-1 by
recombinant vaccinia viruses.
DT 9412
AU Grigoriev VB; Gibadulin RA; Liendeman LF; Sazykin AY; D.I. Ivanovsky
Institute of Virology, Rus. Acad. Med. Sci.; Moscow.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0054). Unique
Identifier : AIDSLINE ICA10/94369281
AB OBJECTIVE: To study assembly of virus like particle (VLP) in process of
expression of gag-pol and gag-pol-env sequences we constructed
recombinant vaccinia virus (VV) strains (RVV). METHODS: RVVs were
constructed by insertion of the gag-pol and gag-pol-env sequences into
VV and termed as RV-1 and RV-2. The expression of HIV-1 proteins by RVVs
was analyzed by immunoblotting and electron microscopy. (EM) RESULTS:
RV-1 synthesized p55, p41 and p24 polypeptides in cells. P17 was
presented in minor quantaties. This strain didn't form VLP in CV-1 and
Hep-2 cell cultures. RV-2 synthesized p160gag, p55gag, 3-4 proteins of
p50-53gag, as well as p41gag ahd p17gag. P24 was present in minor
quantaties. In CV-1 cells gag-proteins didn t form VLP, however in Hep-2
cells muture and immuture VLP can be found by EM. In the cells are
co-infected with RV-1 and RV-2 all above mentioned proteins and VLP were
found. CONCLUSION: The formation of VLP by RV-2 depends on type of cell
culture and level of synthesis of p17. In the cells are co-infected with
two strains, each strain proteins are processed separetely from each
other.
DE Cell Line Gene Expression Gene Products, env/BIOSYNTHESIS/GENETICS
Gene Products, gag/BIOSYNTHESIS/GENETICS Gene Products,
pol/BIOSYNTHESIS/GENETICS *Genes, env *Genes, gag *Genes, pol Human
HIV Antigens/BIOSYNTHESIS/GENETICS HIV-1/GROWTH &
DEVELOPMENT/*GENETICS/METABOLISM Recombination, Genetic Vaccinia
Virus/GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).